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facial roller This fact is likely to limit also the maximum survival rate for homozygous mice treated by any therapy.Suitability of adenoviral and AAV vectors for infection of the target tissuesAs gene therapy vectors were to be administered into the amniotic fluid, components of which may inhibit infectivity of the virus,33, 34 we first tested the stability of reporter gene vectors, AdLacZ and AAVLacZ, in amniotic fluid. In vitro, both vector types were able to infect different human cell lines in the presence of fresh human amniotic fluid (10 with good efficacy, although in pure amniotic fluid the infectivity of the adenoviral vector was slightly reduced, while AAV mediated gene delivery proved to be unaffected (data not shown).Following vector application in vivo, nuclear targeting galactosidase activity was observed in periderm, epidermis and mucous membranes of the oral cavity, oesophagus and trachea of treated animals, but not in parenchymatous organs of these mice. The adenoviral vector infected preferentially the foetal epidermis (Figure 2a), whereas AAVLacZ infected murine keratinocytes poorly (data not shown). facial roller

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